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. 2019 Feb 17;18(3):e12906. doi: 10.1111/acel.12906

Figure 4.

Figure 4

DAF‐18/PTEN binds KIN‐4 to affect the longevity of daf‐2 mutants. (a) Percent lifespan changes by knocking down each of 21 genes that encode proteins that bound the PDZ domain of KIN‐4 in rrf‐3(pk1426) [rrf‐3(−)] and rrf‐3(pk1426); daf‐2(e1370) [rrf‐3(−); daf‐2(−)] mutants. Orange circles indicate the effects of RNAi clones on lifespan. Blue circles indicate RNAi clones that displayed bigger lifespan‐decreasing effects on rrf‐3(−); daf‐2(−) mutants than on rrf‐3(−) worms. A red circle indicates an RNAi clone that further increased the long lifespan of rrf‐3(−); daf‐2(−) mutants. Error bars represent SEM of mean survival times from two or three independent RNAi lifespan experiments. (b−d) daf‐18 (b), mel‐11 (c), and mig‐6 (d) RNAi clones had bigger lifespan‐shortening effects on rrf‐3(−); daf‐2(−) mutant than on rrf‐3(−) animals. (e) rps‐0 RNAi treatment further increased the long lifespan induced by daf‐2(−) mutation in an rrf‐3(−) background. See Supporting information Table S7 for experimental repeats and statistical analyses