Figure 6.

The interaction between KIN‐4 and DAF‐18 is crucial for longevity caused by IIS reduction. (a) Expression pattern of wild‐type [daf‐18 WT], the last four amino acid deletion mutant DAF‐18 transgene [daf‐18 Δ4C] and the last 12 amino acid deletion mutant daf‐18 transgene [daf‐18 Δ12C], fused with mCherry in the posterior intestine cells. (b) daf‐18 WT fully suppressed lifespan reduction by daf‐18(nr2037) [daf‐18(−)] mutation in daf‐2(e1370) [daf‐2(−)] animals. (c) Two independent lines of daf‐18 Δ4C only partially increased the shortened lifespan of daf‐2(−); daf‐18(−) mutants. (d) daf‐18 Δ12C had small lifespan‐increasing effects on the shortened lifespan of daf‐2(−); daf‐18(−) animals. See Supporting information Table S3 for statistics and additional repeats