Inhibition of Akt aggravates, whereas inhibition of GSK-3β or caspases protects against, AILI. (A) Wt mice were injected with vehicle (2% EtOH/PBS) or Akt inhibitor (triciribine at 1 mg/kg) at 5 hours post-APAP, and ALT was measured at 24 hours post-APAP (mean ± SEM; n = 6; *P vs. APAP 1 vehicle). (B) Sdc1–/– mice were injected with vehicle (PBS) or LiCl (200 mg/kg) at 5 hours post-APAP, and ALT was determined at 24 hours post-APAP (n = 5; *P vs. APAP 1 vehicle). (C) Sdc1–/– mice were administered vehicle (EtOH/PBS) or Ac-DEVD-CMK (25 mg/kg) at 3 hours post-APAP, and ALT was determined at 24 hours post-APAP (mean ± SEM; n = 5; *P vs. APAP 1 vehicle).