Fig 6. Real-time transcription of hormonal biosynthesis pathway genes following A. nodosum extract application in tomato and sweet pepper plants.

Plant tissue was collected at time points (0, 12, 24, 48, 72 & 96h) after seaweed extract application and relative qPCR done to monitor transcripts for (A) IPT (B) IAA (C)Ga2Ox2 genes. The data was normalized using the house keeping gene β-actin. Data represents fold change in comparison to the control. The data are averages ± SD for 2 independent trials with 6 replicates. The data was analysed by repeated measures ANOVA at 95% confidence interval to study the effects of time and treatment. The data was also analysed by Student’s t-test to examine the significance of seaweed extract versus control at each time point independently.