Figure 3.

Manifestation of multiple reactive configurations as a function of temperature for investigated PETNR variants. For variants manifesting MRCs, the relative amplitude of the slow kinetic phase (k_obs2) during FMN reduction of PETNR variants by NADPH and NADH increases with temperature. The relative amplitude is reporting on the change in amplitude corresponding to the slow kinetic phase out of the total change in amplitude measured during FMN reduction. For L25A variant, the amplitude value at 40 °C represents the sum of two slow kinetic phases (k_obs2 and k_ob3, see Tables S5 and S10).