(A) Isg15 and Ifit1 levels in whole blood measured by qPCR. (B) pDC were gated as CD11b-PDCA-1+CD11c+ cells. (C) Mice were injected with DOTAP-CpG-C mixture and serum was collected 3 hours post injection. Each symbol represents an individual mouse (n=3–8 per group) from 3 independent experiments, mean +/− SEM are shown. **p < 0.01, ***p < 0.001, (A) Mann-Whitney test, (B) Kruskal Wallis with Dunn’s multiple comparison post test, (C) Mann-Whitney test.