Figure 2. Effect of paternal deprivation on the percentage of c-fos/OT double-labeled neurons in the PVN in adult mandarin voles (n = 5).
Voles were subjected to interaction with either (A) a juvenile voles or (B) a magic cube. (C, D) Double-immunohistochemical staining of c-fos (red) and OT (blue). Effect of PD on the percentage of neurons double-labeled for OT and c-fos in (E) males and (F) females. Error bars indicate SEM. **p<0.01 vs. object stimulus. ##p<0.01 vs. PC. Two-way ANOVA (factors: treatment × stimulus). PC, biparental care; PD, paternal deprivation.