Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Antibody | anti-oxytocin mouse monoclonal antibody | Millipore | Cat#MAB5296 RRID:AB_2157626 |
concentration: 1:7500 |
Antibody | anti-vasopressin rabbit polyclonal antibody | Millipore | Cat#AB1565 RRID:AB_90782 |
concentration:1:4000 |
Antibody | anti-c-fos rabbit polyclonal antibody | Abcam | Cat#ab190289 RRID:AB_2737414 |
concentration:1:1500 |
Antibody | anti-oxytocin receptor rabbit monoclonal antibody |
Abcam | Cat#ab181077 | concentration:1:2000 |
Antibody | anti-vasopressin receptor 1A (V1aR) goat polyclonal antibody | GeneTex | Cat#GTX89114 RRID:AB_10724608 |
concentration:1:7000 |
Antibody | anti-β-tubulin mouse monoclonal antibody | ComWin Biotechnology | Cat#CW0098M | concentration:1:5000 |
Antibody | anti-rabbit goat conjugated with TRITC | Jackson ImmunoResearch | Cat#111-025-003 RRID:AB_2337926 |
concentration:1:200 |
Antibody | anti-rabbit goat conjugated with DyLight 405 | Jackson Immunoresearch | Cat#111-475-003 RRID:AB_2338035 |
concentration:1:200 |
Antibody | anti-rabbit goat conjugated with DyLight 488 | Boster | Cat#BA1127 | concentration:1:200 |
Antibody | anti-mouse goat antibody conjugated with DyLight 488 | Boster | Cat#BA1126 | concentration:1:200 |
Antibody | anti-rabbit goat conjugated with horseradish peroxidase | ZhongShan Goldenbridge | ZB-2301 RRID:AB_2747412 |
concentration:1:10,000 |
Antibody | anti-mouse goat conjugated with horseradish peroxidase | ZhongShan Goldenbridge | ZB-2305 RRID:AB_2747415 |
concentration:1:10,000 |
Antibody | anti-goat rabbit conjugated with horseradish peroxidase | ZhongShan Goldenbridge | ZB-2306 | concentration:1:10,000 |
Reagent | normal goat serum | Boster | Cat#AR0009 | |
Virus | AAV-Ef1α-DIO-ChR2-mCherry | BrainVTA | Cat#PT-0002 | |
Virus | AAV-Ef1α-DIO-eNpHR3.0-mCherry | BrainVTA | Cat#PT-0007 | |
Virus | AAV-Ef1α-DIO-mCherry | BrainVTA | Cat#PT-0013 | |
Virus | AAV-Oxytocin-Cre | BrainVTA | Cat#PT-0263 | |
Chemical compound, drug | radioimmunoprecipitation assay buffer (RIPA) | Solarbio | Cat#R0010 | |
Chemical compound, drug | enhanced chemiluminescence (ECL) reagent | Millipore | Cat#WBKLS0500 | |
Chemical compound, drug | antifade solution | Boster | Cat#AR1109 | |
Chemical compound, drug | 4',6-diamidine-2'-phenylindole dihydrochloride (DAPI) | Boster | Cat#AR1176 | |
Chemical compound, drug | Cholera Toxin Subunit B (CTB)−594 | Thermo Fisher Scientific | Cat#C34777 | |
Software, algorithm | OBSERVER v5.0 | Noldus | https://www.noldus.com/knowledge-base/observer-50 | |
Software, algorithm | SPSS | IBM | RRID:SCR_002865 | |
Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | |
Software, algorithm | GraphPad Prism 5 | GraphPad | RRID:SCR_002798 | |
Software, algorithm | SuperMaze | Shanghai XinRuan | XR-XJ117 | |
Software, algorithm | SocialScan | Clever Sys | http://cleversysinc.com/CleverSysInc/home/software/socialscan/ | |
Other | PVDF membranes | Millipore | C3117 |