Table 2. Cell Culture Media and Solutions.

#	Name	Details
1	Blocking solution	20% goat serum, 0.3% Triton-X 100 in PBS
2	Fibrinogen stock solution	10 mg / mL fibrinogen in 0.9% (wt/v) NaCl solution in water
3	Human fibroblast growth media	Dulbecco’s Modified Eagle’s medium (DMEM), 10% fetal bovine serum, 1% penicillin-streptomycin
4	Human myoblast differentiation media	Dulbecco’s Modified Eagle’s medium (DMEM), 2% horse serum, 10 μg / mL insulin, 1% penicillin-streptomycin
5	Human myoblast growth media	Ham’s F-10 nutrient mix, 20% fetal bovine serum, 5 ng / mL basic fibroblast growth factor, 1% penicillin-streptomycin
6	Hydrogel mixture	Dulbecco’s Modified Eagle’s medium (DMEM), 4 mg / mL bovine fibrinogen, Geltrex (20% v / v), thrombin (0.2 unit/mg fibrinogen)
7	Milk based blocking solution	5% (wt/v) skim milk (BioShop) in TBST
8	Red blood cell lysis buffer	15.5 mM NH4Cl, 1 mM KHCO3, 10 μM EDTA
9	Tris-buffered saline Tween (TBST)	50 mM Tris (BioShop), 150 mM NaCl (Sigma), 0.1% (v/v) Tween 20 (BioShop)