Table 3. List of primary antibodies.
| # | Antibody | Species | Dilution | Source |
|---|---|---|---|---|
| 1 | Alexa Fluor 647 mouse anti-human CD56 | Mouse | 1:20 | BD Pharmingen |
| 2 | Anti-200 kD neurofilament heavy (SMI-32) | Rabbit | 1:200 | Abcam |
| 3 | Anti-C3c (FITC) | Rabbit | 1:200 | Abcam |
| 4 | Anti-HB9/HLXB9 | Rabbit | 1:100 | Abcam |
| 5 | Anti-vimentin | Rabbit | 1:100 | Abcam |
| 6 | Anti- β -tubulin | Rabbit | 1:5000 | Cell Signaling |
| 7 | DRAQ5 | - | 1:1000 | ThermoFisher |
| 8 | Hoechst 33342 | - | 1:1000 | ThermoFisher |
| 9 | Islet-1 | Goat | 5 μg/ml | R and D systems |
| 10 | NRG1- β1 | Mouse | 1:500 | R and D Systems |
| 11 | Monoclonal anti-β-actin-peroxidase | Mouse | 1:50000 | Sigma |
| 12 | Monoclonal mouse anti-human desmin |
Mouse | 1:100 | Dako |
| 13 | MuSK (PA5-14703) [WB] | Rabbit | 1:1000 | Invitrogen |
| MuSK (PA1-1741) [IF] | Rabbit | 1:50 | Invitrogen | |
| 14 | Myosin heavy chain - embryonic | Mouse | 1:50 | DSHB |
| 15 | Myosin heavy chain - fast | Mouse | 1:50 | DSHB |
| 16 | Myosin heavy chain - slow | Mouse | 1:50 | DSHB |
| 17 | Myosin heavy chain - pan | Mouse | 1:50 | DSHB |
| 18 | Nicotinic acetylcholine receptor β |
Rabbit | 1:2000 | Novus |
| 19 | Nicotinic acetylcholine receptor epsilon |
Rabbit | 1:1000 | Novus |
| 20 | Rapsyn | Mouse | 1:1000 | Abcam |
| 21 | Sarcomeric alpha-actinin | Mouse | 1:200 | Sigma |
| 22 | α-Bungarotoxin, Alexa Fluor 647 conjugate | - | 1:500 | ThermoFisher |