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. 2019 May 14;8:e39905. doi: 10.7554/eLife.39905

Figure 4. Genetic deficiency or pharmacologic depletion of IgE protects against the development of osteoarthritis in mice.

(a–d) Cartilage degradation in medial regions of stifle joints from C57BL/6J IgE-deficient (Igh7-/-, n = 7) and IgE-sufficient (Igh7+/+, n = 6) mice 20 weeks after DMM surgery. Representative safranin-O stained medial stifle joint sections from these mice are shown (a); arrowheads show severe cartilage loss. Quantification of cartilage degradation (b), osteophyte formation (c), and synovitis (d). (e–h) Cartilage degradation in medial regions of stifle joints from C57BL/6J mice subjected to DMM surgery and then treated i.p. with anti-IgE antibody (n = 6) or isotype-matched control antibody (n = 7) 2.5 mg/kg twice per week for 12 weeks. Representative Safranin-O stained medial stifle joint sections from these mice are shown (e); arrowheads show severe cartilage loss. Cartilage degeneration (f), osteophyte formation (g), and synovitis (h) in medial regions of stifle joints from these mice are quantified. Symbols represent scores from individual mice. Bars denote mean ± s.d. *p≤0.05, **p≤0.01, by Mann Whitney test. Scale bars, 200 μm. Scoring of joint pathologies was performed by an investigator blinded to experimental groups. Data are representative of two independent experiments with similar results.

Figure 4.

Figure 4—figure supplement 1. Deficiency of IgE reduces synovitis and osteophyte formation in mice subjected to DMM.

Figure 4—figure supplement 1.

(a) Representative H&E-stained knee joint sections from IgE-sufficient (Igh-7+/+) and IgE-deficient (Igh-7-/-) mice 20 weeks after DMM surgery. (b) Representative H&E-stained knee joint sections from C57BL/6J mice administered with isotype control (IgG1κ), or anti-IgE (2.5 mg/Kg/day) antibodies twice a week for 12 weeks following DMM surgery. Yellow arrows show osteophyte and open arrows show synovial thickening or synovitis. Scale bars, 200μm.
Figure 4—figure supplement 2. Mast cell numbers in DMM joint tissue in IgE deficient and wild-type mice following DMM.

Figure 4—figure supplement 2.

Quantification of mast cells by toluidine blue staining of joint tissue derived from IgE-sufficient (Igh-7+/+) and IgE-deficient (Igh-7-/-) mice 20 weeks following surgical DMM from Figure 4a. Statistical comparisons were performed using Student’s t test (*P <0.05).