Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| sStrain, strain background (C. elegans) |
N2 (wild type) | CAENORHABDITIS GENETICS CENTER (CGC) |
N/A | https://cgc.umn.edu/strain/N2 |
| sStrain, strain background (C. elegans) |
CB1489 (him-8 (e1489)) | CAENORHABDITIS GENETICS CENTER (CGC) | WB Cat# CB1489,RRID:WB-STRAIN:CB1489 | https://cgc.umn.edu/strain/CB1489 |
| Strain, strain background (C. elegans) | CB1370 (daf-2 (e1370)) | CAENORHABDITIS GENETICS CENTER (CGC) |
WB Cat# CB1370, RRID:WB-STRAIN:CB1370 | https://cgc.umn.edu/strain/CB1370 |
| Strain, strain background (E. coli) |
OP50 | CAENORHABDITIS GENETICS CENTER (CGC) | WB Cat# OP50, RRID:WB-STRAIN:OP50 | https://cgc.umn.edu/strain/OP50 |
| Genetic reagent (E. coli) | HT115 (DE3), control bacteria for RNAi |
CAENORHABDITIS GENETICS CENTER (CGC) |
WB Cat# HT115(DE3), RRID:WB-STRAIN:HT115(DE3) |
https://cgc.umn.
edu/strain/HT115(DE3) |
| Genetic reagent (E. coli) | mes-3 RNAi clone | PMID: 12828945 | N/A | Ahringer RNAi library |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/download.html | ImageJ, RRID:SCR_003070 | SeeSupplementary file 8 |
| Software, algorithm | 3D Objects Counter Plugin Imagej | https://imagej.net/3D_Objects_Counter | 3D Objects Counter, RRID:SCR_017066 |
SeeSupplementary file 8 |
| Software, algorithm | 3D ROI Manager |
http://imagejdocu.tudor.lu/doku.php?
id=plugin:stacks:3d _roi_manager:start |
N/A | See Supplementary file 8 |