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. Author manuscript; available in PMC: 2019 May 14.
Published in final edited form as: Nature. 2018 Nov 7;563(7733):646–651. doi: 10.1038/s41586-018-0686-x

Fig. 4 |. Precise template-free correction of pathogenic alleles.

Fig. 4 |

a, Efficient correction of a pathogenic allele to wild-type. b, c, Comparison among pathogenic alleles of observed and predicted frequencies of repair to wild-type genotype (b) and frame (c). d, Wild-type repair frequencies of pathogenic alleles with predicted frequency ≥50% among all major editing outcomes, in mESCs. Dashes indicate means. e, f, For mESCs containing the LDLRdup1662_1669dupGCTGGTGA–P2A–GFP allele, flow cytometric contour plots (e) and fluorescence microscopy (f). Representative data for n = 2 independent biological replicates. Major editing outcomes include +1 to −60 indels.