Extended Data Figure 5: Characterization of Lib-B data including pathogenic microduplication repair in wild-type mESCs, wild-type U2OS cells, and mESCs treated with DPKi3, NU7026, and MLN4924.

a, Box plots of the number of unique high-confidence editing outcomes (see Supplementary Methods) called by simulating data subsampling in data at 2,000 Lib-B target sites in mESCs (combined data from n = 2 independent technical replicates) and U2OS cells (combined data from n = 2 independent biological replicates). In “all”, the full non-subsampled data is presented (see Supplementary Table 2 for read counts). Each box depicts data for 2,000 target sites. The box denotes the 25^th, 50^th, and 75^th percentiles and whiskers show 1.5 times the interquartile range. Outliers are not depicted. b, Frequencies of repair to wild-type genotype at 567 ClinVar pathogenic alleles vs. predicted frequencies in Lib-B in human U2OS cells with Pearson r. c, Frequencies of repair to wild-type frame at 437 ClinVar pathogenic alleles vs. predicted frequencies in Lib-B in human U2OS cells with Pearson r. d, Frequency of pathogenic microduplication repair in wild-type mESCs (n = 1,480 target sites) compared to mESCs treated with MLN4924 (n = 1,569), NU7041 (n = 1,561), and DPKi3 (n = 1,563).