Comparison 5. Subgroup by encoding gene: gene therapy versus no gene therapy control.

Outcome or subgroup title	No. of studies	No. of participants	Statistical method	Effect size
1 Amputation‐free survival	4	756	Odds Ratio (M‐H, Random, 95% CI)	1.68 [0.75, 3.76]
1.1 FGF encoding	2	650	Odds Ratio (M‐H, Random, 95% CI)	1.34 [0.46, 3.91]
1.2 VEGF encoding	1	54	Odds Ratio (M‐H, Random, 95% CI)	3.95 [1.07, 14.65]
1.3 HGF encoding	1	52	Odds Ratio (M‐H, Random, 95% CI)	1.56 [0.38, 6.38]
2 Complete ulcer healing	5	238	Odds Ratio (M‐H, Fixed, 95% CI)	2.16 [1.02, 4.59]
2.1 FGF encoding	1	125	Odds Ratio (M‐H, Fixed, 95% CI)	1.48 [0.54, 4.04]
2.2 HGF encoding	3	92	Odds Ratio (M‐H, Fixed, 95% CI)	5.06 [1.23, 20.84]
2.3 HIF‐1α encoding	1	21	Odds Ratio (M‐H, Fixed, 95% CI)	0.57 [0.04, 8.05]
3 Amputation (above‐ankle amputation of the index limb)	11	1336	Odds Ratio (M‐H, Fixed, 95% CI)	1.06 [0.77, 1.46]
3.1 FGF encoding	2	650	Odds Ratio (M‐H, Fixed, 95% CI)	1.07 [0.75, 1.55]
3.2 HGF encoding	3	125	Odds Ratio (M‐H, Fixed, 95% CI)	1.14 [0.27, 4.81]
3.3 VEGF encoding	5	523	Odds Ratio (M‐H, Fixed, 95% CI)	0.75 [0.33, 1.69]
3.4 HIF‐1α encoding	1	38	Odds Ratio (M‐H, Fixed, 95% CI)	6.33 [0.33, 122.40]
4 All‐cause mortality	12	1685	Odds Ratio (M‐H, Fixed, 95% CI)	0.93 [0.66, 1.31]
4.1 FGF encoding	2	650	Odds Ratio (M‐H, Fixed, 95% CI)	1.05 [0.69, 1.60]
4.2 HGF encoding	3	185	Odds Ratio (M‐H, Fixed, 95% CI)	0.73 [0.22, 2.50]
4.3 VEGF encoding	5	523	Odds Ratio (M‐H, Fixed, 95% CI)	0.81 [0.32, 2.09]
4.4 HIF‐1α encoding	2	327	Odds Ratio (M‐H, Fixed, 95% CI)	0.68 [0.28, 1.63]