Fig. 4.

Increased monocyte recruitment in vivo by IL-7. Matrigel plugs containing 15μg/ml IL-7 or PBS injected into flanks of mice fed high fat diet were collected after 3 days and subjected to digestion for cell recovery. The recovered cells were analyzed for fluorescent staining and flow cytometry. Gating for monocytes as depicted in Supplemental Fig. 2A. Gating for T cells as depicted in Supplemental Fig. 3A. Results are expressed as mean percentage of total cell infiltrates of each Matrigel plug. *p = 0.04 t-test; N = 6 each.