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. 2019 May 14;9:7381. doi: 10.1038/s41598-019-43689-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of miR-126 inhibition on HIF-1α and Hsp90 protein levels in early passage HUVECs. (A) Representative HIF-1α western blot in early passage HUVECs transfected with negative control (NC) inhibitor, miR-126-3p strand, miR-126-5p strand or both sequence inhibitors, miR-126-3p plus miR-126-5p for 72 hours. Equal protein loading was confirmed probing with GAPDH. (B) The graphs present densitometric band analysis normalized to GAPDH in arbitrary units (AU). The data represent means ± SD. n = 3.Control vs. miR-126-transfected early passage HUVECs cells. *p < 0.05 and **p < 0.01. (C) Representative Hsp90 western blot in early passage HUVECs transfected with negative control (NC) inhibitor, miR-126-3p strand, miR-126-5p strand or both sequence inhibitors, miR-126-3p plus miR-126-5p for 72 hours. Equal protein loading was confirmed probing with GAPDH. (D) The graphs present densitometric band analysis normalized to GAPDH in arbitrary units (AU). The data represent means ± SD. n = 3.