Table 2.

TSP3 active site mutant halo assay results.

Wella	Mutation	Halo Formationb
1	Wild-type	++
2	E362Q	+
3	D383N	++
4	D426N	+
5	E362Q and D383N	+
6	E362Q and D426N	−
7	D383N and D426N	+
8	E362Q, D383N, and D426N	−
9	PBS Control	−

^aWell numbering corresponds to Fig. 6.

^bSymbols are as follows: −, No observable halo;+, 0–1.5 cm halo diameter; ++, >1.5 cm halo diameter.