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. 2019 May 14;9:7346. doi: 10.1038/s41598-019-43813-3

Figure 3.

Figure 3

Sec31 is distant from most of the COPII components at ER exit sites. HeLa cells were fixed and costained with anti-Sec12 (green), anti-cTAGE5 (red) and anti-Sec31 (blue) (a); anti-Sec12 (green), anti-Sec16 (red) and anti-Sec31 (blue) (b); anti-Sec23 (green), anti-cTAGE5 (red) and anti-Sec31 (blue) (c); anti-Sec23 (green), anti-Sec16 (red) and anti-Sec31 (blue) (d) antibodies. 3D triple -color observation by SCLIM is shown. Right, magnifications of images on the left with 2D triple-color projection. The length indicates the scale of each unit.