Figure 3.
Sec31 is distant from most of the COPII components at ER exit sites. HeLa cells were fixed and costained with anti-Sec12 (green), anti-cTAGE5 (red) and anti-Sec31 (blue) (a); anti-Sec12 (green), anti-Sec16 (red) and anti-Sec31 (blue) (b); anti-Sec23 (green), anti-cTAGE5 (red) and anti-Sec31 (blue) (c); anti-Sec23 (green), anti-Sec16 (red) and anti-Sec31 (blue) (d) antibodies. 3D triple -color observation by SCLIM is shown. Right, magnifications of images on the left with 2D triple-color projection. The length indicates the scale of each unit.