Figure 1.

LAMP amplification initiation, cycling and elongation. Strand invasion of double stranded DNA with LAMP and displacement primers initiates the LAMP reaction. Positions of primer recognition sites are shown in red for displacement primers, green for LAMP primers and blue for Loop primers. LAMP initiation leads to the formation of dumbbell-like structures with cycling between the two forms and elongation into cauliflower-like structures with multiple loops. The fluorophore is shown unquenched attached to the FIP primer and quenched by proximity to guanine bases due to fluorescence resonance energy transfer (FRET).