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. 2019 May 14;9:7400. doi: 10.1038/s41598-019-43817-z

Figure 2.

Figure 2

Quenching of fluorescence. LAMP amplification with 35S promoter primers substituted with a FIP primer labelled with JOE and LoopF primer labelled with FAM. Dilution series of a linear plasmid template and with additional no template control. Quenching of the FAM signal from the FAM-LoopF primer (A) and quenching of the JOE signal from the JOE-FIP primer (B). Four replicates for each dilution of template and for NTCs. Average Ct (cycle threshold set at −0.05 or −0.07) against logarithmic scale of the template dilutions for JOE-FIP (C) and FAM-LoopF assays (D). Three out of four samples were positive at 50 copies, one out of four samples were positive at 5 copies. The R2 values with the highest concentration of template removed were 0.9868 and 0.9795 for JOE-FIP and FAM-LoopF respectively.