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. 2019 May 14;9:7400. doi: 10.1038/s41598-019-43817-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection with JOE-FIP and SYTO9. LAMP amplification with 35S promoter primers of a linearised plasmid template (1) quenched fluorescence in presence of JOE-FIP primer with SYTO9 using yellow channel detection of JOE, (2) fluorescence of same samples with green channel detection of SYTO9, (3) fluorescence with unmodified primers with SYTO9 only (green channel). (A) Normalised fluorescence truncated from 60 minute assay, (B) melt curve analysis, (C) average Ct plotted against template concentration on a log scale and (D) summary of replicate Ct values, average Ct and standard deviation (SD) for each dilution. NTCs (red lines) were negative. Light blue lines: 500000 copies; orange: 50000 copies; dark blue: 5000 copies; brown: 500 copies; green: 50 copies.