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. 2019 May 14;9:7400. doi: 10.1038/s41598-019-43817-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Non-specific primer interactions detected with SYTO9 and JOE-FIP. Panel (A) shows the normalised fluorescence from SYTO9 detection and (C) shows the consecutive JOE-FIP fluorescence for NTCs. Corresponding melt curve analysis shown in panels (B,D). Negative results and SYTO9 detected positives are highlighted in red, JOE-FIP detected positives are highlighted in blue. One negative and all positives were examined on an agarose gel (E) for amplicons and the JOE-FIP detected positives showed a ladder pattern associated with LAMP amplification.