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. 2019 May 14;9:7400. doi: 10.1038/s41598-019-43817-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Carrier DNA in LAMP amplification with SYTO9 and JOE-FIP detection. Panels (1A to C) represent amplification with the 35S promoter primers of a low concentration of denatured maize genomic DNA (5.0 percent maize GM event Bt11). Panels (2A to C) represent amplification of the same sample with the NOS terminator primers. Panels (A) show the amplification frequency for increasing concentrations of carrier DNA. Cycle threshold set for all assays at −0.005. Panels (B) show the average Ct values and panels (C) shows the fastest Ct values with increasing concentrations of salmon sperm carrier DNA. Green indicates SYTO9 derived values, blue indicates JOE-FIP derived values and red indicates ‘false positive’ results derived from the difference between the SYTO9 and JOE-FIP values. In panels 1C and 2C the SYTO9 and JOE-FIP values are identical.