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. 2019 May 14;9:7400. doi: 10.1038/s41598-019-43817-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Duplexed LAMP reaction with ADH1 and 35Sp detection. LAMP amplification and detection of 20 copies of denatured Bt11 maize genomic DNA with ADH1 LAMP primers with FAM labelled FIP and 35Sp LAMP primers with TAMRA labelled FIP. Blue indicates template amplification and orange denotes the NTCs. The (DUPLEX) column shows the detection of two LAMP reactions in each tube. Other columns show the individual LAMP reactions and the detection channels of the thermocycler; FAM-FIP detected on green channel only and TAMRA-FIP by the orange channel.