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. 2019 May 14;9:7353. doi: 10.1038/s41598-019-43744-z

Figure 4.

Figure 4

Acquired motor memory is retrieved without mGluR1 in PCs. (A) Development of CR% in mGluR1 ON/ON mice (n = 10), mGluR1 ON/OFF mice (n = 10), and mGluR1 OFF/OFF mice (n = 10). Top panel indicates experimental design for the timing and period of eyeblink conditioning in the three mouse gropes. Gray boxes indicate the timing and period of DOX administration. Mouse age is indicated above the schedules. In mGluR1 ON/OFF mice, mGluR1 was expressed without Dox in the first conditioning (days 1–7), but the expression was blocked with Dox in the second conditioning (days 57–61). The CR% for mGluR1 ON/OFF mice was as high as those for mGluR1 ON/ON mice on day 57, even though no mGluR1 protein remained in PCs, demonstrating that mGluR1 in PCs is dispensable for the expression of once acquired eyeblink memory. Controls were mGluR1 ON/ON and mGluR1 OFF/OFF mice, and the data for these mice are identical to those shown in Fig. 3. *p < 0.05 (ON/ON vs. ON/OFF). ††p < 0.01, †††p < 0.001 (ON/OFF vs. OFF/OFF). (B) Averaged EMG amplitudes on days 7, 57, and 61. All EMG amplitudes obtained in one session (100 trials) were summed, representing the overall response pattern. (C) The intraday CR% for mGluR1 ON/OFF mice on days 7 and 57. On day 57, the CR% for mGluR1 ON/OFF mice was as high as that for mGluR1 ON/ON mice in all blocks. Data are presented as in Fig. 3. (D) Expression of mGluR1 in mGluR1 ON/OFF mice. Coronal sections from mGluR1 cKO mice at different time points after the start of Dox administration were stained with antibody against mGluR1. (E) Immunoblotting of cerebellar proteins from mGluR1 cKO mice30 (ON/ON and ON/FF) with anti-mGluR1 and anti-β-actin antibodies. Proteins from a wild-type mouse (WT), a transgenic mouse harboring L7-mGluR1a transgene (rescue)26 and a global mGluR1 KO mouse (KO)16 are identical to those shown in Fig. 3G. The blots cropped from different parts of a same gel were separately shown with a white space. All lanes contain 10 µg of protein. Scale bar: 1 mm. Data are represented as mean ± SEM.