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. 2019 May 14;9:7372. doi: 10.1038/s41598-019-43758-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional characterisation of HKlincR1 (AC093323.3) (A) Genomic location of HKlincR1. Representative image of H460 RNA-seq track encompassing 50 kb up and downstream of HKlincR1 was plotted using the UCSC genome browser. Non-targeting control track (siNT) was shown in black and siHKlincR1 track was shown in red. (B) RT-PCR and qRT-PCR (C) of HKlincR1 transcript levels across 20 different normal human tissue types. β-actin was used as a positive control. Cropped gel images for HKlincR1 and β-actin were shown in (A). Full length gels are presented in Supplementary Fig. S4C. (D) Protein-protein interaction network of differentially expressed genes following HKlincR1 knockdown. Significant functional modules were identified using ClusterONE⁷² in Cytoscape⁷¹ (p-value < 0.01; Minimum cluster size >4). Biological processes significantly enriched in each cluster were identified using BiNGO⁷³ in Cytoscape (Adjusted p-value < 0.05). (E–G) qRT-PCR analysis of HKlincR1-dependent genes relative to β-actin following HKlincR1 knockdown in H460, H1755 and A549 lung cancer cells. Non-targeting (NT) siRNA was used as a control.