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. 2019 May 8;10:579. doi: 10.3389/fpls.2019.00579

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Copyright © 2019 Wu, Holtkamp, Wairich and Frei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genotyping and semi-quantitative RT-PCR analysis of the Tos17 insertional lines for potassium ion channel OsAKT1. (A) Schematic representation of the OsAKT1 gene structure and Tos17 insertions in two different lines, NG1928 and NC2778. (B) Genotyping results of T2 plants of NG1928 and NC2778 line. (C) Semi-quantitative RT-PCR of OsAKT1 in NG1928 and NC2778 segregation lines. Rice ubiquitin gene (OsUBQ5) was used as a reference. The sequences of the primers are listed in Supplementary Table S1. P1_F, P1_R: gene-specific primers for NG1928; P2_F, P2_R: gene-specific primers for NC2778; Tos_tail6: Tos17 primer; M: DNA marker; WT: Nipponbare; AZ-1: azygos line of NG1928; AZ-2: azygos line of NC2778.