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. 2019 May 8;10:579. doi: 10.3389/fpls.2019.00579

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Copyright © 2019 Wu, Holtkamp, Wairich and Frei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Fe-transport genes in roots of OsAKT1 lines exposed to Fe toxicity (17.9 mM Fe²⁺ for 5 days). (A) OsFRDL1, (B) OsIRT1, (C) OsYSL2, and (D) OsYSL15 expression in the root were analyzed with quantitative RT-PCR. Vertical bars represent mean values ± standard errors (N = 3). Sequences of the primers used in the test were listed in Supplementary Table S1. T: treatment; G: genotype; T × G: treatment by genotype interaction; ^∗∗∗ P < 0.001, n.s: not significant; AZ: azygos lines.