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. 2019 May 8;10:553. doi: 10.3389/fphys.2019.00553

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Copyright © 2019 García-Prieto, Gil-Ortega, Vega-Martín, Ramiro-Cortijo, Martín-Ramos, Bordiú, Sanchez-Pernaute, Torres, Aránguez, Fernández-Alfonso, Rubio and Somoza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Representative immunoblots of p-AMPK/AMPK and β-actin expression in PBMCs from non-obese, obese and post-surgery patients. Diagram bars show the result of densitometric analysis of p-AMPK/AMPK immunoblots expressed as a percentage of p-AMPK/AMPK in the non-obese group. (B) Correlation between p-AMPK/AMPK and BMI. (C) Representative immunoblots of SIRT1 and β-actin expression in PBMCs from non-obese, obese and post-surgery patients. Diagram bars show the result of densitometric analysis of SIRT1/β-actin immunoblots expressed as a percentage of SIRT1/β-actin in the non-obese group. (D) Correlation between SIRT1/β-actin and BMI. Data are expressed as mean ± SEM of ≥ 7 determinations per group. ^#p < 0.05 and ^##p < 0.01 compared with the non-obese group. ^∗p < 0.05 and ^∗∗∗p < 0.001 compared with the obese group.