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. 2019 May 8;9:141. doi: 10.3389/fcimb.2019.00141

Figure 3.

Figure 3

Vi brings about molecular rearrangements in the membrane raft. (A) Cells were left untreated or treated with Vi for 1 h at 4 or 37°C. Lipid rafts were prepared from lysates prepared from these cells by sucrose density gradient centrifugation. Fractions were absorbed on to a nitrocellulose membrane and probed with HRP-conjugated Cholera Toxin B (HRP-CTB). (B) Untreated or Vi-treated cells (for 1 h at 37°C) were infected with S. Typhimurium (100 moi for 30 min). Fractions 4, 5, and 6 were run in a 10% SDS-polyacrylamide gel, transferred to a nitrocellulose membrane and probed with antibodies to prohibitin and Rac1. Blots were developed using ECL. Data are representative of 2 independent experiments.