FIG 5.

Effects of GP78 on EGFR signaling and DUSP1 expression. Huh7 cells were serum starved for 2 h, stimulated with EGF at the indicated time points, and harvested for analyses of the indicated proteins by Western blotting (shown at the top). (A) GP78-v5-overexpressing and vector control Huh7 cells. (B) Huh7 cells infected with shRNA-GP78-containing lentiviruses or control viruses. Downregulation of GP78 by shRNA was confirmed by IP and Western blotting, and the impacts of its downregulation on the indicated proteins are shown. (C) GP78 KO and control cells. p-Y, tyrosine antibody was used to detect EGF-dependent tyrosine phosphorylation. (Top) The impacts of its knockout on the indicated proteins are shown. (A to C, bottom) Fold changes were normalized relative to total forms or actin (n = 3; means ± SD are shown; *, P < 0.05). Numbers to the left of the gels (B and C) are kilodaltons.