Table 1. Species and their GenBank accession numbers used in this study.

Species	Accession number	Source
Opsariichthys group
Parazacco spilurus	KF971863	Chang et al. 2016
Candidia barbata		This study
Candidia pingtungensis	KT725246	Yin et al. 2015
Nipponocypris koreanus	KJ427719	Chen et al. 2016a
Nipponocypris sieboldii	AB218898	Saitoh et al. 2006
Nipponocypris temminckii	KM213515	Chen et al. 2016b
Zacco acanthogenys	KT290890	Yin et al. 2015
Zacco platypus	AP012115	Miya et al. 2015
Opsariichthys acutipinnis	KT725245	Yin et al. 2015
Opsariichthys bidens	DQ367044	Wang et al. 2007
Opsariichthys chengtui	KT725244	Yin et al. 2015
Opsariichthys evolans		This study
Opsariichthys pachycephalus		This study
Opsariichthys uncirostris	AB218897	Saitoh et al. 2006
Xenocyprididae
Hemigrammocypris rasborella	AP011422	Tang et al. 2010
Metzia longinasus	KF955011	Ma & Luo 2016
Metzia mesembrinum	NC_023797	Yuan et al. 2016
Metzia formosae	NC_022458	Lin et al. 2015
Metzia lineata	NC_031541	GenBank
Aphyocypris chinensis	AB218688	Saitoh et al. 2006
Aphyocypris kikuchii	JX184925	Jang-Liaw et al. 2013b
Yaoshanicus arcus	AP011398	Tang et al. 2010
Nicholsicypris normalis	AP011396	Tang et al. 2010
Pararasbora moltrechti	JX311312	Jang-Liaw et al. 2013a
Macrochirichthys macrochirus	NC_015551	GenBank
Paralaubuca typus	AP011211	Saitoh et al. 2011
Ctenopharyngodon idella	EU391390	Wang et al. 2008
Elopichthys bambusa	AP011213	Miya et al. 2015
Hypophthalmichthys molitrix	KJ729094	Farrington et al. 2015
Hypophthalmichthys nobilis	KJ746959	Farrington et al. 2015
Squaliobarbus curriculus	KC351187	Liu et al. 2013
Xenocypris argentea	AP011283	Mayden et al. 2009
Xenocypris davidi	KF039718	Liu 2014
Ischikauia steenackeri	NC_008667	Saitoh et al. 2006
Chanodichthys mongolicus	KF826087	Wei et al. 2016
Chanodichthys ilishaeformis	NC_029722	Li et al. 2016
Chanodichthys dabryi	NC_021418	Zhang et al. 2014
Culter erythropterus	NC_024749	Chen et al. 2016
Culter recurviceps	NC_024277	GenBank
Culter mongolicus	AP009060	Saitoh et al. 2006
Parabramis pekinensis	KF857485	Duan et al. 2016
Megalobrama amblycephala	NC_010341	GenBank
Megalobrama pellegrini	NC_026458	Liu et al. 2016
Hemiculter bleekeri	NC_029831	GenBank
Hemiculter leucisculus	NC_022929	GenBank
Hemiculter eigenmanni	NC_029388	GenBank
Cprinidae
Cyprinus carpio	AP017363	Mabuchi 2016
Danionidae
Rasbora vaterifloris	NC_015531	Tang et al. 2010
Rasbora lateristriata	NC_032723	Kusuma & Kumazawa 2016
Rasbora trilineata	NC_025336	Ho et al. 2016
Rasbora steineri	NC_020005	Chang et al. 2013
Danio dangila	NC_015525	Tang et al. 2010
Danio erythromicron	AP011419	Tang et al. 2010
Danio rerio	NC_002333	Broughton et al. 2001
Acheilognathidae
Acheilognathus macropterus	NC_013711	Hwang et al. 2014
Acheilognathus typus	NC_008668	Saitoh et al. 2006
Rhodeus ocellatus	NC_011211	He et al. 2008
Rhodeus lighti	NC_024885	Wang et al. 2016
Rhodeus sinensis	NC_022721	Yang et al. 2015
Rhodeus shitaiensis	NC_022690	Li et al. 2015
Tanakia limbata	NC_025515	Luo et al. 2016
Tanakia lanceolata	NC_024566	Xu et al. 2016
Gobionidae
Hemibarbus barbus	NC_008644	Saitoh et al. 2006
Squalidus gracilis	NC_024561	Liu et al. 2016
Abbottina rivularis	NC_023781	He et al. 2013
Gobio gobio	NC_008662	Saitoh et al. 2006
Rhinogobio typus	NC_024423	Yan et al. 2016
Gnathopogon elongatus	NC_008649	Saitoh et al. 2006
Sarcocheilichthys variegatus microoculus	NC_004694	Saitoh et al. 2003
Leucisidae
Leuciscus burdigalensis	NC_029426	Hinsinger et al. 2015
Acrocheilus alutaceus	AP012086	GenBank
Cyprinella lutrensis	NC_008643	Saitoh et al. 2006
Macrhybopsis storeriana	NC_030485	Gaughan et al. 2016
Tincidae
Tinca tinca	AB218686	Saitoh et al. 2006
Tanichthys micagemmae	NC_031631	GenBank
Tanichthys albonubes	NC_015539	GenBank