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Isolation and characterisation of cardiac c-kit cells. (A) Immunocytochemistry staining of cardiac c-kit cells. CCs expressed c-kit, cardiac transcription factors (GATA4, NKX2.5), pluripotent marker (SOX2) but negative for mast cell marker (Tryptase). (B) Dexamethasone-directed cardiac differentiation of CCs for 21 days showed positive expression of cardiac markers (cTnI), smooth muscle actinin (SMA) and endothelial marker (vWF). Nuclei were stained with DAPI (Blue). Scale bar = 100 μm.
