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. 2019 Apr 12;38(10):e100785. doi: 10.15252/embj.2018100785

Figure EV3. Size‐exclusion chromatography.

Figure EV3

  1. Size‐exclusion chromatography of 500 μg nickel‐affinity‐purified Mpc1/Mpc3 hetero‐complex. In the A280 profiles, the Mpc1/Mpc3 hetero‐complex was present in a symmetrical peak.
  2. Size‐exclusion chromatography of 150 μg nickel‐affinity‐purified Mpc3. Mpc3 produced a peak similar to that of Mpc1/Mpc3.
Data information: Insets in (A and B) show peak fractions collected and analysed by SDS–PAGE and visualised by Coomassie Blue stain.