Figure EV1. The TssJLM complex exhibits a C5 symmetry in Escherichia coli BL21 and in EAEC.

1. Slice (13.8 nm) through an engineered BL21 minicell with a diameter of ˜450 nm. Scale bar 100 nm.
2. Slice (9.7 nm) showing a side view of a TssJLM particle embedded in the cell envelope of a BL21 minicell. The particle spanned the periplasm and resembled an inverted letter Y.
3. Slice (9.7 nm) showing a top view of a TssJLM particle embedded in the membrane of a BL21 minicell. The particle resembles a 5‐branched star.
4. Slice (9.7 nm) showing a side view of a particle embedded in the cell envelope of a BL21 ghost cell.
5. Slice (9.7 nm) showing a top view of a particle embedded in the cell envelope of a BL21 ghost cell.
6. Slice (9.7 nm) showing a side view of a particle embedded in the cell envelope of a FIB‐milled BL21 cell.
7. Slice (13.8 nm) showing a top view of a particle embedded in the cell envelope of a FIB‐milled BL21 cell.
8. Slice (9.9 nm) showing a side view of a particle embedded in the cell envelope of an EAEC cell in which TssJLM was heterologously overexpressed.
9. Slice (9.9 nm) showing a side view of a particle embedded in the cell envelope of a wild‐type EAEC cell.
10. Slice (9.9 nm) through the distorted periplasm of an EAEC in which TssJLM was heterologously overexpressed. The particles seen in side view were detached from the OM but still attached to the periplasmic side of the IM.

Data information: Slices in panels (B–J) show side views (upper rows) and top views (lower rows) of TssJLM embedded in the cell envelope of BL21(DE3) (expressing TssJLM) and EAEC cells, as indicated by red arrows. The outer and inner membranes are indicated (OM and IM). Scale bars 10 nm.