Table 2.
Cryo‐EM data collection, refinement and validation statistics
| TssJLM core complex (EMD‐0264) (PDB 6HS7) | TssJLM complex (EMD‐0265) | TssJLM C1 complex (EMD‐0266) | TssJLM base complex (EMD‐0267) | |
|---|---|---|---|---|
| Data collection and processing | ||||
| Magnification | 120,000× | 120,000× | 120,000× | 120,000× |
| Voltage (kV) | 200 | 200 | 200 | 200 |
| Electron exposure (e–/Å2) | 120 | 120 | 120 | 120 |
| Defocus range (μm) | 0.4–5 | 0.4–5 | 0.4–5 | 0.4–5 |
| Pixel size (Å) | 1.24 | 1.24 | 1.24 | 1.24 |
| Symmetry imposed | C5 | C5 | C1 | C5 |
| Initial particle images (no.) | 167,825 | 167,825 | 167,825 | 167,825 |
| Final particle images (no.) | 36,828 | 36,828 | 36,828 | 36,828 |
| Map resolution (Å) | 4.6 | 4.9 | 7.9 | 17 |
| FSC threshold | 0.143 | 0.143 | 0.143 | 0.143 |
| Map resolution range (Å) | 3.9 and 18 Å | 3.8–33 Å | – | – |
| Tomography | ||||
| Number of grid points | ||||
| Voxel size | ||||
| Projections | ||||
| Refinement | ||||
| Initial model used (PDB code) | 4Y7O | |||
| Model resolution (Å) | ||||
| FSC threshold | 4.6 Å | |||
| Model resolution range (Å) | ||||
| Map sharpening B factor (Å2) | ||||
| Model composition | ||||
| Non‐hydrogen atoms | 52,890 | |||
| Protein residues | 6,905 | |||
| Ligands | N/A | |||
| B factors (Å2) | ||||
| Protein | ||||
| Ligand | N/A | |||
| R.m.s. deviations | ||||
| Bond lengths (Å) | 0.006 | |||
| Bond angles (°) | 0.989 | |||
| Validation | ||||
| MolProbity score | 1.92 | |||
| Clashscore | 6.39 | |||
| Poor rotamers (%) | 0.04 | |||
| Ramachandran plot | ||||
| Favoured (%) | 89.46 | |||
| Allowed (%) | 10.54 | |||
| Disallowed (%) | 0 | |||