Table 2.

Primers used in vector construction and real-time qPCR.

Names of primers	Sequences (5′−3′)	Usage
SP 1_F	ATGGTCGCAGAGCTTGTC	Strain validation
SP 1_R	CAGCACAATAGAACACCAGAC	Strain validation
avrA_F1	TCTTAGATCCAGGTACCTGGTCCAGCAACAGATCTCCGAT	avrA knockout
avrA_R1	ATTATTGTCCATTGCATTGGCCGGAGTGTTAATCATGGTG	avrA knockout
avrA_F2	ATTCCTGTCCATTGCATTGGTTGTATGTCGCCCCTAAAGG	avrA knockout
avrA_R2	CTGCAGAACCAGAGCTCTGGAGCGGGACAAACTGTTCG	avrA knockout
avrA_F0	TCTCCAGGCAAATACGCTCG	avrA knockout
avrA_R0	GAGTTCAGGCTGGTCGACTG	avrA knockout
mtarP1:	CGAGATGGATGGGGTAGACGCAGACAACATGCATCCACTTGCGG	Site mutation of p1
mtarP1cr	CCGCAAGTGGATGCATGTTGTCTGCGTCTACCCCATCCATCTCG	Site mutation of p1
mtarP2:	GACGGTCTCAACATGCATCCAGAACGCGATGATCCGCTCCCGATCGC	Site mutation of p2
mtarP2cr:	GCGATCGGGAGCGGATCATCGCGTTCTGGATGCATGTTGAGACCGTC	Site mutation of p2
11665F	GGTCTTGCCTACGTGCTTGA	Real-time PCR
11665R	CACACTCGCCTTTGGCATTG	Real-time PCR
11670F	AGCTGCAATTGAAGATCAGCG	Real-time PCR
11670R	TGTGTTCTTGATGCCAGCCG	Real-time PCR
11675F	AACGTGTTCCGGGTAACCTC	Real-time PCR
11675R	CGACCGATGGAGTCACGAAT	Real-time PCR
11680F	CGCAAACATGGTCTGGTGAG	Real-time PCR
11680R	CGGCCAAGGTAATATCCCCG	Real-time PCR
proiscR_F	ACGCGTCGACGCGGTATTCTGACCTCGGTG	Fusion vector
proiscR_R	CTCGCCCTTGCTCACCATCAGTCTCATGTGCCTTACCG	Fusion vector
eGFP_F	CGGTAAGGCACATGAGACTGATGGTGAGCAAGGGCGAG	Fusion vector
eGFP_R	TCGCGGATCCTTACTTGTACAGCTCGTCCA	Fusion vector
pUC19_F	AATGCAGCTGGCACGACAGG	Fusion vector
pUC19_R	CCATTCAGGCTGCGCAACTG	Fusion vector
proavrA_F	CGGGGTACCCAGTCGTTGCTCCATGGCGG	Fusion vector
proavrA_R	CCGGAATCCGGTCGCCAACTTCTACATCT	Fusion vector
proavrA comF	CGGGGTACCAAACAGTAGCCAGGGACCGAG	Fusion vector
proavrA comR	GGCCGGAATTCGCCTTTATCGCCGATCTGC	Fusion vector
pBBR1MCS-2 F	GGCACCCCAGGCTTTACACT	Complement plasmid validation
pBBR1MCS-2 R	GATGTGCTGCAAGGCGATTAAG	Complement plasmid validation