Figure 1.
Mouse experimental workflow. Groups of mice were immunized by intramuscular electroporation with 50 μg of either pV-ZME or pV and were boosted twice at 3-week intervals. Splenocytes were obtained 1 week after the last immunization, and sera were collected 3 weeks after the last immunization. Subsequently, vaccinated mice were challenged with 1 × 106 PFU of DENV1, 200 PFU of DENV2, 1 × 106 PFU of DENV3, or 1 × 105 PFU of DENV4. At days 1, 2, 4, 6, and 8 post challenge, the peripheral blood of mice was collected to evaluate the dynamics of viremia. Body weight changes and survival rates were observed for 21 consecutive days after challenge.