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Immunization of mice with the Zika DNA vaccine pV-ZME confers a reduction in DENV viremia and cross-protection against challenge with four serotypes of DENV (A–D). The kinetics of viremia was evaluated in peripheral blood on days 1, 2, 4, 6, and 8 after DENV1–4 challenge. (E–H) Body weight changes and (I–L) survival rates were monitored daily for 21 consecutive days (n = 14). The results are expressed as the mean values ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001.
