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. 2019 Apr 25;8(4):560. doi: 10.3390/jcm8040560

Figure 2.

Figure 2

UNR/CSDE1 promotes metastatic phenotype in CRC. (A) CSDE1 mRNA expression of eight stage II tumor samples, 16 stage III tumor samples, and six stage IV tumor samples by qRT-PCR. (B) UNR protein expression of primary and metastatic CRC cell lines and parental and colospheres derived CRC cell lines. (C) CSDE1 mRNA expression of CRC derived cell lines from (B) grouped by origin. (D) Two different siRNAs of csde1 (si15373 and si15374) were used to downregulate UNR protein expression in DLD1 and SW620 CRC derived cell lines. We verified UNR expression levels by western blot at 24 and 48 h after transfection. (E) MTS assay showed that both DLD1 and SW620 cell lines decreased cell viability at 48 h after UNR downregulation. (F) Microscope images of wound healing assay showed a reduced cell migration after UNR downregulation in both cell lines. Representative images taken at 6, 12, and 24 h after scratching. Arrows represent distance between cell migration heads. (G) Cell invasion assays performed in Boyden chamber showed that UNR silencing decreased invasion ability of both cell lines. UNR/Actin ratio is represented under Actin protein bands. All assays were performed with two different siRNA sequences. (* p < 0.05; ** p < 0.01).