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. 2019 Mar 30;8(4):436. doi: 10.3390/jcm8040436

Figure 2.

Figure 2

Upregulation of HO-1 suppresses TNF-α-induced ICAM-1 expression on human pulmonary alveolar epithelial cells (HPAEpiCs). (A) Cells were infected with an indicated multiplicity of infection (MOI) with either an empty adenovirus (Adv) or Adv-HO-1 for the indicated time intervals. (B) HPAEpiCs were infected with 20 MOI of empty Adv or Adv-HO-1 for 36 h, followed by 30 ng/mL TNF-α for 10 h. (C,D) HPAEpiCs were pretreated with 20 μg/mL hemoglobin (Hb) for 1 h and then treated with 50 μM CORM-2 for 6 h, followed by incubation with 30 ng/mL TNF-α for 10 h. (A–C) The levels of HO-1, ICAM-1, and β-actin protein were determined by western blot. (D) The adhesion of THP-1 cells was measured. Data are expressed as mean ± SEM, n = 3. # p < 0.01, as compared with the respective groups as indicated.