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. 2019 Mar 30;8(4):436. doi: 10.3390/jcm8040436

Figure 3.

Figure 3

CORM-2 induces HO-1 expression via Nox/ROS. (A) HPAEpiCs were pretreated with APO, DPI, or NAC for 2 h and then incubated with CORM-2 for 16 h. The protein levels of HO-1 and β-actin were determined by western blot. (B) Cells were pretreated with APO (100 μM), DPI (10 μM), or NAC (10 mM) for 2 h and then incubated with CORM-2 for 6 h. The mRNA expression of HO-1 was determined by real-time PCR. (C) Cells were treated with CORM-2 for the indicated time intervals. Nox activity and ROS generation were measured. (D) Cells were pretreated with APO (100 μM) or DPI (10 μM) and then incubated with CORM-2 for 2 h or 4 h. Nox activity and ROS generation were measured. Data are expressed as mean ± S.E.M. of three independent experiments (n = 3). # p < 0.01, as compared with the cells exposed to the vehicle alone (C) or CORM-2 alone (A,B,D).