Figure 5.

CORM-2 induces c-Src/EGFR-dependent HO-1 expression. (A) HPAEpiCs were pretreated with PP1 or AG1478 for 1 h and then incubated with CORM-2 for 16 h. The protein levels of HO-1 and β-actin were determined by western blot. (B) Cells were pretreated with PP1 (10 μM) or AG1478 (10 μM) for 1 h and then incubated with CORM-2 for 6 h. The mRNA expression of HO-1 was determined by real-time PCR. (C) Cells were transfected with scrambled, c-Src, or EGFR siRNA and then incubated with CORM-2 for 16 h. The levels of c-Src, EGFR, HO-1, and β-actin protein were determined by western blot. (D) Cells were transfected with scrambled, c-Src, or EGFR siRNA and then incubated with CORM-2 for the indicated time intervals. The levels of c-Src, EGFR, p47, phospho-c-Src, phospho-EGFR, phospho-p47, and β-actin protein were determined by western blot. Data are expressed as mean ± S.E.M. of three independent experiments (n = 3). ^# p < 0.01, as compared with the cells exposed to CORM-2 alone or compared between the indicated groups.