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. 2019 Mar 30;8(4):436. doi: 10.3390/jcm8040436

Figure 6.

Figure 6

CORM-2 induces HO-1 expression via PI3K/Akt. (A) HPAEpiCs were pretreated with LY294002 or SH-5 for 1 h and then incubated with CORM-2 for 16 h. The protein levels of HO-1 and β-actin were determined by western blot. (B) Cells were pretreated with LY294002 (10 μM) or SH-5 (1 μM) for 1 h and then incubated with CORM-2 for 6 h. The mRNA expression of HO-1 was determined by real-time PCR. (C) Cells were transfected with scrambled or Akt siRNA and then incubated with CORM-2 for 16 h. The levels of Akt, HO-1, and β-actin protein were determined by western blot. (D) Cells were pretreated without or with LY294002, SH-5, PP1, AG1478, APO, DPI, or NAC for 1 h and then incubated with CORM-2 for the indicated time intervals. The protein levels of phospho-Akt and β-actin were determined. Data are expressed as mean ± S.E.M. of three independent experiments (n = 3). # p < 0.01, as compared with the cells exposed to CORM-2 alone or compared between the indicated groups.