Figure 1.

Investigated microfluidic trapping and cultivation devices: (A) mother machine (MM) with growth channel connected to two supply channels, (B) monolayer growth chamber (MGC) connected to one supply channel, and (C) negative dielectrophoresis (nDEP) within supply channel. Cells are indicated by green dots and fluid flow by blue arrows. (D–F) illustrate respective CFD geometries. (F) features an extended channel after the cell trap to reduce the impact of computational artefacts at the exit boundary. Red insets show microcolonies in geometries (D,E), geometry (F) contains a single cell.