Figure 4. Laser ablation‐induced ethylene responses are partially dependent on calcium increases.

• A–D
  XYZ maximum projection images of ACS6::NLS‐3xVenus ethylene biosynthesis marker line in the Arabidopsis root after laser ablation with or without GdCl₃ (50 μM). Time points in hours (h) at the top right corner of each frame. White arrowheads indicate position of cortex cell ablation. Representative pictures are shown.
• E, F
  Signal intensity quantification and number of cells with positive nuclear (NLS‐3xVenus) signal increases after ablation in control, but increases are reduced upon GdCl₃ (50 μM) inhibitor treatment (*P < 0.05, the significance was determined by t‐test, data pooled from three independent experiments with n = 10 roots each; error bars indicate mean value with 95% CI).

Data information: Scale bar, (A–D) 100 μm.