Figure 8. Cyst nematode damage leads to ethylene response.

• A, B
  Tile‐scan with 4D projection (xyzt) images (A, B) monitoring ethylene biosynthesis by ACS6::NLS‐3xVenus prior to cyst nematode (Heterodera schachtii) infection in 5‐day‐old roots. (A) Control scan demonstrating weak ACS6 response over 14 h in the non‐invaded root section (n = 9). (B) ACS6 expression after nematode intrusion into root tissues (n = 10). White arrowheads indicate position of nematodes, and black arrows indicate time frame when nematode invasion of the root was first observed.
• C
  ACS6::NLS‐3xVenus ethylene biosynthesis marker line quantification 3 h after nematode intrusion into root tissues (***P < 0.004, the significance was determined by t‐test; error bars indicate standard error, data pooled from three independent experiments).

Data information: Scale bar: (A, B) 100 μm. See also Movies EV2, EV3 EV4 and EV5.