Fig. 2.

Lipid storage, mitochondrial function and glycogen content in gastrocnemius muscle of Stk25 transgenic and wild-type mice. (a) Representative immunofluorescence images stained with lipophilic Nile Red dye (green). Scale bar, 15 μm. Histograms show quantification of Nile Red staining. (b) Representative immunofluorescence images stained with MitoTracker Red (yellow). Scale bar, 15 μm. Histograms show quantification of MitoTracker staining. (c) Representative histochemical staining for NADH, SDH and COX activities. Scale bar, 50 μm. (d) Representative electron micrographs from cross-sections showing lipid droplets (red arrows) and mitochondria, which are swollen (red arrowheads), display disarrayed cristae and reduced electron density of the matrix (open arrowheads), and internal vesicles (green arrowhead). Scale bar, 2 μm. (e) Representative images stained with PAS (scale bar, 15 μm) and quantification of PAS staining. In (a, b and e) data are mean ± SEM from six mice per genotype. *p < 0.05; **p < 0.01. HFD, high-fat diet; TG, transgenic; WT, wild-type